Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Upgradeable modules are also available including western blot, cell imaging, and fast. Choose cables that meet the requirement described in this appendix. e. 1. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Five preferred detection modes. 6 x 10-15. Lift up the lid over the chamber to insert or remove a cuvette. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. SpectraMax M5. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seMolecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available) $34,000. Bioz Stars score: 86/100, based on 1 PubMed citations. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. 6 x 10-15. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellNanoRacks, LLC is installing a new, reconfigured Molecular Devices’ SpectraMax M5e multi-mode microplate reader on the International Space Station to perform experiments in microgravity. We would like to show you a description here but the site won’t allow us. It was difficult and time consuming to undertake quality, repetitive research. Choose from a three- (M3), four- (M4), orSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. Table of Contents. At the low light level (approaching the Lower. Both instruments demonstrated good performance for the assay. SpectraMax ABS Plus SpectraTest ABS2 v1. Jeffrey Manber, Managing Director of NanoRacks said, "Having the SpectraMax M5e Reader on-orbit will allow researchers to undertake research with real-time results. SpectraMax Mini: 17. 1. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax® L (software version 5. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In this. Dual monochromators for assay flexibility. No sabemos si este producto volverá a estar disponible, ni cuándo. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. 508/20. Your lab might even have one already. M3-M5e_manual. 1. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. 3. Please see the exact video and photos below. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFor calcium content quantification, separate plates were used. 90 0. 90-0083 series): Excitation 530 nm,. The following day, calcium content was quantified by the QuantiChrom™ Calcium Kit (BioAssay Systems) by reding absorbance at 612 nm using the Spectramax M5e reader (Molecular Devices). The Quant-iT RiboGreen RNA Assay Kit, when run on a SpectraMax microplate reader with fluorescence detection mode and SoftMax Pro Software, is a rapid, sensitive detection method for RNA. Examples: “Press carefully to engage,” “Do not. The following document is intended to demonstrate setup of this instrument. View the Molecular Devices website. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. measured using a microplate reader (SpectraMax M5e, Molecular Devices, San Jose, CA, USA) with excitation and emission at 494 and 520 nm, respectively, and calibrated against a standard curve for FITC. 0. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. M5e = 174nM SpectraMax M5e delta F % Paradigm = 18nM M5e = 19nM Figure 1. Note: In this user guide, all references to SpectraMax Multi-Mode. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. Choose from a three- (M3), four- (M4), orMicroplate handling system for the spectramax paradigm multi-mode detection platform (110 pages). 2 x 10-14. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 5 mP standard deviation at 1 nMOEM: Molecular DevicesMaterial: SpectraMax M5e Common Name: Microplate Spectrophotometer Item Weight: 36 Serial Number: MVE06577 SKU#: Inv-00352193. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. This document is provided to customers who have purchased Molecular Devices, Inc. The optical systemsProducts : SpectraMax M5/M5e reader Can BRET be read on the SpectraMax M5? BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. The resulting purple crystals were dissolved in solubilization buffer and analyzed by spectrophotometry at 490 nm, using a reference of 650 nm in a microplate reader (SpectraMax M5e, MD, USA. 01 1 100 10000 1000 2000 3000 SpectraMax Paradigm SpectraMax M5e [cAMP] nM delta F % EC 50 Values Paradigm = 18nM M5e = 19nM Variation of Integration Time-12 -10 -8 -6 -4 5000 10000 15000 20000 25000 50 s delay 400 50 s. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 1. The optical systemsThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). spr SpectraMax Plus 384. 1. SUNNYVALE, CALIF. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFigure 2: Expression of mCherry with (improved BBa_K1141000) and without (BBa_K1141000) the translation enhancing 5’ untranslated region was measured (Ex: 587 nm, Em: 610 nm) over a period of 6 hours after induction with 0. 8 x 10-15. This document is provided to customers who have purchased Molecular Devices, Inc. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. The SpectraMax M2 and M2e are versatile and reliable. Other technologies such as imaging. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. Your lab might even have one already. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Cell monolayers were decalcified with 0. Z1. cAMP HiRange 0. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Gemini XPS. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart Features We would like to show you a description here but the site won’t allow us. If you love a cozy, comedic mystery, you'll love this 'whodunit' adventure. View the Molecular Devices website. KeySpan adapter for SoftMax Pro and SpectraMax readers. Path Check Pathlength Measurement The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The user manual is an indispensable documentation for of any product. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. At the American Heritage School, located in Plantation, Florida, students used a SpectraMax® M5e to conduct scientific research during their first Annual Summer Science Program. Conquer Scientific provides laboratories around the world high quality, low-cost pre-owned laboratory instruments. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. The following document is. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. The plate reader. 485. SpectraMax® M5. HTRF-compatible SpectraMax Multi-Mode Readers. 40266 Email: drugdiscoverytech@lifetech. SpectraMax M5e: 118. Introduction. 00We would like to show you a description here but the site won’t allow us. 3. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. For more information please visit us at: SpectraMax®i3Multi-ModeMicroplateReader* SpectraMax®i3xMulti-ModeMicroplateReader*. HTRF cAMP calibration curve HTRF cAMP calibration curve measured on the SpectraMax i3 (red circles), SpectraMax. The mirror set. The system had to pass vibration load, zero gravity, and temperature fluctuation testings. Skatron Skanwasher 300 Washer. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The following document is intended to demonstrate setup of this instrument. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. Optimal instrument settings are the same for all three readers. All associated StakMax® stackers. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 0001 0. A fluorescence microscope with high-throughput screening capabilities (Axio Observer. Proceed with the following steps to optimize the instrument: 1. 1)M5e = 174nM SpectraMax M5e delta F % Paradigm = 18nM M5e = 19nM Figure 1. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SoftMax Pro 软件模板设置之一标准曲线的绘制以及样品浓度的自动计算. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. AquaMax® 4000. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Jul 13, 2023 10am PT Date Format. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. M5/M5e Multi-Mode Microplate Reader SpectraMax. SpectraMax Absorbance, Fluorescence, Luminescence, and Multi-Mode Readers. SpectraMax Glo and DuoLuc. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. 5 mM IPTG using a SpectraMax M5E. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices SpectraMax™ M5 Microplate Reader w/ Warranty (Svc Contract Available) $29,500. View the Molecular Devices website. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. #M5e) Methods Microplate reader compatibility test. The drawing shows a standard microplate of 15 mm height covered with a lid. 8 x 10-15. Wide. SpectraMax M5e: 118. The chamber contains springs that position the cuvette in the proper alignment for a read. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart . M5 reader microplate m3 spectramax molecular devices llc m5e biocompare quote fluorescenceMolecular devices spectramax plus 384 user manual pdf download Spectramax® m2/m2e multimode microplate readers, with softmax® proMolecular devices spectramax m5 microplate reader labx contract svc warranty available manufacturer. 0001 0. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. 40266 Email: drugdiscoverytech@lifetech. 5. 1. 0 Application Area:. -- JULY 19, 2016Cables. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. Table of Contents. SpectraMax Injector Cartridge with SmartInject™ Technology. Supplier: Molecular Devices. SpectraMax i3 reader SpectraMax Paradigm reader SpectraMax M5e reader cAMP EC 50 (nM) 19. 314 low lamp level warning. 2 x 10-14. Multi-Mode Microplate Readers. Categories:. For example, microplates containing different samp les can be read using the same orSpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. Bioz Stars score: 86/100, based on 1 PubMed citations. 1 & 7. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. SpectraMax M5e: 118. The optical systemsThe BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Bookings are free, but you will need to use iLab to book. Multimode Readers Comparison Chart. 92 0. -- JULY 19, 2016 Cables. No disponible por el momento. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. For more sample throughput, the system canSetup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax M5e Multi. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. The following document is intended to demonstrate setup of this instrument and provide representative5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Meso Scale Discovery 1250 Sector Imager 2400 $ 2,500. It was also read on the monochromator-based SpectraMax® M5e Multi-Mode Microplate Reader using optimized wavelengths. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. 现有酶标仪的客户基本都是需要进行数据分析，可能习惯使用Excel、Prism等软件，对我们SoftMax Pro的数据分析功能不了解，事实上大家可使用SoftMax Pro对数据进行完整处理并不需要再导入第三方. Molecular Devices SpectraMax® series microplate readers. HTRF assay performance on the SpectraMax M5e reader is demonstrated here with two different HTRF assay kits, cAMP dynamic 2 and IP-One. Pick the best speed, sensitivity, and price for your needs. 1. book Page 1 Wednesday, July 7, 2010 2:09 PM. SpectraMax M2. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. 5 mP standard deviation at 1 nM SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Settings are for each instrument are shown in Table 1. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. M5e: W = 15. Delivers single mode reader performance in one multimode reader package. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. 1. Status: Reviewer Member since: 2020 Organization: IIUM Ease of use After sales service Value for money Great results, easy life, bright future. View the Molecular Devices website. com. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 标准功. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. 4, Z’ = 0. Jeffrey Manber, Managing Director of NanoRacks said, "Having the SpectraMax M5e Reader on-orbit will allow researchers to undertake research with real-time results. elegans, and Alcanivorax borkumensis. 3. Molecular Devices. SUNNYVALE, Calif. The BRD4 bromodomain 1 TR-FRET assay was performed as described in the assay kit manual. The schematic of the UED setup is shown as Fig. Configure your microplate reader. 8. A two-fold dilution series of JQ1 (in DMSO) starting from 1 μM was prepared. assays on the SpectraMax® Paradigm® microplate detection platform. 1. In this product video, see how the SpectraMax M5 quickly reads samples. com 10. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The guide also covers the software features, the specifications, and the accessories of the systems. ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. #M5e) Methods Cell preparation. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. 4. The cellular imaging systems Molecular. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. These robust readers have been placed in labs from Antarctica to the. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The Spectr. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax® M5e Multi-Mode Microplate Reader (Molecular Devices) Assay setup and detection. SpectraMax Mini: 17. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. Under these assay conditions, up to 75. reader (TUNE) SpectraMax M5e. Toxicokinetics studies on cynomolgus monkey. HTRF is compatible with a large range of available microplate readers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SpectraMax M5e Multi-Mode Microplate Reader. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Ends from. The dual monochromator optics allow. 6 x 10-15. book Page 21 Wednesday, July 7, 2010 2:09 PM. com A. Using. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The absorbance of the wells containing only the primary antibodies but no antigen was used as the background value. 1. Molecular Devices SpectraMax M5e Microplate Reader. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. SPECTRAMAX XS XENON REPLACEMENT BULB FOR MOLECULAR DEVICES SPECTRAMAX XPS XENON, SPECTRAMAX XS. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Relative fluorescence units (RFU) were converted to units of NADPH concentration (μmol/L) using a standard curve of NADPH. 5, Z’ = 0. 1, left panel) and individual wells (Fig. $. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellDNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. 8 x 10-15. • SpectraMax M5e Multi‐Mode Microplate Reader • FlexStation® 3 Multi‐Mode Microplate Reader Overview. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 8. 1 19. 6 M HCl for 24 h. 305 reference level saturation. In this application note we show how the SpectraMax ® i3, SpectraMax ® Paradigm ®, and SpectraMax ® M5e Multi-Mode Microplate Readers are used to perform robust, high-throughput HTRF ® assays with excellent Z’ factors and highly reproducible EC 50 values. The following document is intended to demonstrate setup of this instrument. 3. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. Add to cart. 1. 8. book Page 1 Wednesday, July 7, 2010 2:09 PM. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Homogeneous Time-Resolved Fluorescence (HTRF) . Page 8 FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Based on the SpectraMax M5e instrument platform, the FlexStation 3 instrument can address detection modalities including absorbance, fluorescence intensity, fluorescence polarization, time-resolved fluorescence, and luminescence. 1. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 8 x 10-15. Explore PDF for more details. Molecular Devices SpectraMax M5e Microplate Reader. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. This assay may also be detected on the SpectraMax ® i3 and SpectraMax ® M5e Multi-Mode Microplate ReadersThe SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 7°C (gradient heating design). FlexStation™ II. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 91. 0200-0729. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The donor and acceptor can be used to label a variety of biomolecules, for. 2222 Fifth Street. D 1 1. 40266 Email: drugdiscoverytech@lifetech. The detector is a CCD array with 512 pixels in x and 512 pixels in y. After washing, the chromogenic substrate was added, and the OD was read using a SpectraMax M5e microplate spectrophotometer (Molecular Devices) at 450 nm. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Real-time fluorescence signals were recorded using CFX96 thermocycler (Bio-Rad). With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Our absorbance plate readers feature our PathCheck Sensor. Upgradeable modules are also available including western blot, cell imaging, and fast. The validation kit included individual tubes of diluted fluorophores: FAM, HEX, and FAM+HEX. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMulti-mode Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. SpectraMax Gemini EM/XPS. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. , contact Molecular. . Choose cables that meet the requirement described in this appendix. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. FlexStation™ III. Paradigm: W = 21.